Application Notes
Explore our latest insights from PICO
Quantification of extracellular vesicles (EVs) with PICO>
In this application note we detected and quantified extracellular vesicles (EVs) from serum or cell supernatant using PICO technology. The EVs were measured with two different detection strategies: 1) using an anti-CD63 antibody, halved and labeled with two different PICO Labels, we quantified CD63+ EVs, while 2) using both anti-CD63 and anti-CD9 antibodies, we quantified CD63/CD9+ EVs. These findings highlight the versatility of PICO in sensitive and accurate detection of various protein targets.
PICO vs. Western Blot>
In this application note we compared the sensitivity of PICO to western blot. We chose to detect the breast cancer biomarker HER2/ErbB2 in two different breast cancer cell lines, BT474 and MCF7. Through our analysis, we found that PICO demonstrated approximately 200-fold higher sensitivity compared to western blot. These findings further support the advantages of using PICO for sensitive and accurate protein detection.